【作者】 晁志； 谢晖； 霍克克； 潘胜利；

【Author】 CHAO Zhi 1 XIE Hui 2 HUO Ke-ke 3 PAN Sheng-li2 (1 School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515; 2 School of Pharmacy, Fudan University, Shanghai 200032; 3 School of Life Sciences, Fudan University, Shanghai 200433)

【机构】 南方医科大学中药学院； 复旦大学药学院生药教研室； 复旦大学生命科学研究院；

【摘要】 目的比较研究中国柴胡属药用植物rDNAITS序列的差异和规律,寻找可准确鉴定柴胡属药材的分子性状。方法PCR扩增ITS序列,测序,DNAssist version 2.2软件进行序列比对,并计算同源性。结果ITS序列与外类群ITS序列比对同源性均小于75%,序列间两两比对同源性均大于87%,大于99%可作为鉴定指标。结论ITS序列作为中国柴胡属药用植物鉴定性状具有一定实用性和可靠性。更多还原

【Abstract】 Objective: To study on the patterns of the rDNA ITS sequences variation of medicinal plants from genus Bupleurum in China, and to find DNA molecular markers for identification of crude drugs from the genus. Methods: ITS region were amplified by PCR and sequenced. The sequences were analyzed by DNAssist version 2.2. Results:Homologous alignment indicated that consanguinity of ITS sequences between genus Bupleurum and outgroups was lower than 75%, and that within genus Bupleurum was higher than 88%. In case of same species, it’s higher than 99%. Conclusion: ITS sequences can be applied as reliable molecular markers in the identification of Radix Bupleuri.更多还原