【作者】 宋霞； 刘新刚； 莫炜； 严小敏； 戴林森；

【Author】 Song Xia~1 Liu Xingang~1 MoWei~2 Yan Xiaomin~1 Dai Linsen~1 1 Research Center of Analysis & Measurement,Fudan University,Shanghai,200433 2 The Key Laboratory of Molecular Medicine,the Ministry of Education,Fudan University,Shanghai,200032.P.R.China

【机构】 复旦大学分析测试中心； 复旦大学教育部分子医学重点实验室；

【摘要】 <正>新型重组双功能水蛭素由66个氨基酸残基组成,相对分子量约为7KDa。样品是通过重组 DNA 技术将精氨酸—甘氨酸—天冬氨酸(RGD)序列融合在野生型水蛭素的合理部位,经表达和纯化后得到的高纯度重组水蛭素蛋白质。活性实验表明它具有抗凝血酶和抗血小板聚集的双重功能,临床上可用于治疗不稳定型心绞痛,深静脉血栓和预防急性心肌梗死等疾病,因此对它的结构与功能关系的研究引起了人们广泛的兴趣。更多还原

【Abstract】 The new type of recombinant RGD-Hirudin studied in this paper consists of 66 amino acid residues with～7KDa molecular weight.Employing recombinant DNA technique,it’s constructed by introducing the Arg-Gly-Asp(RGD)sequence to the proper site of wild-type hirudin.Then high purified recombinant RGD-Hirudin was obtained by expression and purification.It has been proved that the recombinant RGD-Hirudin has dual functions of anti-thrombin and anti-platelet aggregation by active assays.更多还原