【作者】 肖超妮； 张许； 刘买利； 常建华；

【Author】 XIAO Chaoni~(1,2) ZHANG Xu~1 LIU Maili~(1*) CHANG Jianhua~2 (1 State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics,Wuhan Institute of Physics and Mathematics,Wuhan,430071) (2 Department of Chemistry,Northwest University,Xi’an,710069)

【机构】 波谱与原子分子物理国家重点实验室(中国科学院武汉物理与数学研究所)； 化学系西北大学；

【摘要】 <正>在生物分子的 NMR 研究中,消除水峰的脉冲会使与水具有相同或相近化学位移的信号峰同时得到抑制,结果导致共振信号的丢失和信息量的减少．这些丢失的信号可以用 RECUR 方法得到恢复。RECUR 利用极化转移的原理,把远离溶剂峰的核磁共振信号通过偶极-偶极相互作用或标量偶合传递到信号被抑制了的质子,使其共振峰得以恢复。该方法已经成功地在一维~1H NMR 谱和二维 J 分解谱中得到应用．更多还原

【Abstract】 We present here the extension of the RECUR（recovery of under-water resonances）for two-dimensional correlation experiments.The proposed pulse sequence is shown in Figure I.After high-efficiency water resonance suppression using double WATERGATE（W5）,RECUR sequence is applied to reestablish the resonances close to,or under,the water peak through magnetization transfer via either scalar or dipolar coupling.The magnetization is then allowed to free processing under chemical shift and J-coupling during t₁ period.Before data acquisition,A 90° hard pulse, MLEV pulse train or spin-lock sequence can be used achieve the magnetization transfer in the manner exactly the same as the conventional COSY,TOCSY and ROSY experiments.更多还原