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用固相萃取-核磁共振谱法对光活布洛芬在大鼠体内代谢产物的研究

Metabolites of Chiral Ibuprofen as Studied by Solid Phase Extraction Chromatography-Nuclear Magnetic Resonance Spectroscopy in Rat Urine

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【作者】 孔漫杨春张守仁徐瑞明司伊康

【Author】 Kong Man Yang Chun Xu Ruiming Zhang Shouren Si Yikang (Institute of Materia Medica,The Chinese Academy of Medical Sciences,Beijing 100050)

【机构】 中国医学科学院、中国协和医科大学药物研究所

【摘要】 <正>目前,高分辨核磁氢谱已经有可能从尿、血浆等生物样品中直接检测和鉴定药物及其代谢产物(1,2)。固相萃取技术可以把大部分内源性物质除去,采用梯度洗脱的方法可以把代谢混合物分成若干部分,以利于药物代谢产物的结构解析。

【Abstract】 Ibuprofen(2-[4’-isobutylphenyl]-propionic acid,IBP) is an important nonsteroidal anti- inflammatory drugs with antipyretic activities.We study the metabolites ofchiral IBP in rat urine by SEP-PAK column and NMR.A stepwise gradient of methanol and water was applied and fractions were scanned by proton NMR.The main metabolites are 2’- hydroxy-IBP、2’-hydroxy-IBP-glucuronide、2’-carboxy-IBP and IBP-glucuronide in the spectra of(±)-IBP which shows no differece with that by Wilson I D.And we didn’t discover taurine-IBP and glycine-IBP which are derived from the transformation from (- )-IBP to (+)-IBP.There is no main difference in the total spectrums of racemic and chiral IBP.But in the spectra of 10% and 60% fractions of R-IBP,some peaks appeared.It demonstrates that R-IBP did not inverted to S-IBP entirely,and it has some new metabolites.

  • 【会议录名称】 第十届全国波谱学学术会议论文摘要集
  • 【会议名称】第十届全国波谱学学术会议
  • 【会议时间】1998-09
  • 【会议地点】中国山东青岛
  • 【分类号】R96
  • 【主办单位】中国物理学会波谱学专业委员会(Committee of the Magnetic Resonance Spectroscopy Chinese Physical Society)
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