【作者】 张友杰； 阎江丽； 袁汉珍； 张晓东； 毛希安； 沈联芳；

【Author】 Youjie Zhang~* Jiangli Yan~(+*) Hanzhen Yuan~+ Xiaodong Zhang~+ Xian Mao~+ Liangfang Shen~+ +Laboratory of Magnetic Resonance and Atomic and Molecular Physics,Wuhan Institute of Physics,Academy of Sciences,Wuhan 430070 *The center of Analysis and Testing,Central China Normal University,Wuhan 430070

【机构】 华中师范大学分析测试中心； 中科院武汉物理研究所,波谱与原子分子物理国家重点实验室； 中科院武汉物理研究所波谱与原子分子物理国家重点实验室；

【摘要】 对于双螺旋DNA,残基内的标量偶合作用,如C残基上相邻碱基质子C5H与C6H、T残基上C5甲基质子与邻位C6上的质子、糖环上相邻质子之间的相互作用等均在COSY和NOESY谱上产生强的交叉峰。而残基之间的交叉弛豫则使每个碱基质子（C6H或CBH）都与5′端相邻糖环的质子及自身糖环上的质子产生NOE交叉峰。本文用COSY和NOESY谱归属了八聚DNA d[GGTATACC]₂质子的化学位移,如表1所示。并在此基础上,用¹³C-¹H逆检测相关谱（HMQC）确定了该DNA碳的化学位移（表2）。并经进一步计算确定了DNAd[ GGTATAGC]₂在D₂O溶液中的三维空间结构。更多还原

【Abstract】 For the double helix of DNA,scalar coupling interactions between intra-residual protons,such as H5 and H6 in the residual of C,CH₃ at C5 and H6 in T,H1′and H2′or H2″and so on,rise cross-peaks in COSY as well as in NOESY spectra.Otherwise,as a result of direct or secondary magnetization transfer,a base proton can exhibit NOE interaction with the sugar protons of its own group and of the 5′neighboring nucleotide.So the resonances from protons of d[GGTATACC]₂ are identified with 2D NOESY and COSY spectra,and the chemical shifts of protons are listed in tab.1.Data in tab.1,except that of H5′and H5″,are in accordance with the data front the ¹³C-¹H HMQC spectrum.更多还原