【作者】 丁继贞； 陈润生；

【Author】 Ding Ji-zhen Chen Run-sheng (NMR Group.Institute of Biophysics,Academic Sinica,Zhang Guan Cun,Beijing,China,100080)

【机构】 中国科学院生物物理所,核磁共振组；

【摘要】 用~1H同核相关谱(COSY)和欧伏毫斯效应相关谱(NOESY、ROESY)对甲硫脑啡肽(Met~5-enkephalin)在DMSO-d6和D20中的溶液构象进行了研究;并用计算机进行三维空间结构的模拟。甲硫脑啡肽在DMSO-d6和D20中主要采取扩展形式,是以两个独立的扩展肽骨架成对称的反平行β-折迭。接近生理pH时一部分分子可能呈两性形式,由于静电相互吸引使二体的头尾接近。Tyr、Phe和Met的侧链均具有高的灵活性,可能与选择性受体结合有关。更多还原

【Abstract】 The conformations of Met~5-enkephalin in solution of DMSO-d6 and D20 have been studied by ~1H HOMCOR and NOESY ROESY.The three-dimension structures of the molecules also have been simulated by SGI-graphic workstation.Met~5-enkephalin predominantly adopted extended conformation in DMSO-d6 and D20.There are two independent extended peptide backbone on the form of a symmetric unit,which is antiparallelβ-sheet structure.Near physiological pH a part of the enkephalin molecules may be become zwitterion.Owing to electrostatic interaction the head and end of the dimer may also be closed.The side chains of Tyr, Phe and Met are high flexibility.It is perhaps relevant to select the receptor binding sites.更多还原