【作者】 杨培强； 黄永仁； 周元聪；

【Author】 Yang Pei Qiang Huang Yong Ren (Analytical Center,East China Normal University,ShangHai 200062)

【机构】 华东师范大学分析测试中心； 中国科学院上海生物化学研究所；

【摘要】 <正>蝮蛇毒液中提取的酸性磷脂酶是124肽蛋白质,在消化中起着重要的作用,经生化研究证明有预防凝血的功能,在医学上有重要的价值。通过蛋白质的降解分析,其一级序列已分析清楚,但其高级结构仍未报导研究过。我们更多还原

【Abstract】 Acidic Phospholipases A2 from the venom of Agiskistrodonhalys pallas is a protein which has 124 peptides.Althouth its sequence was determinded by Edman degradation,its secondary and tertiary structure has not been solved.This review shows the assighment of spin systems about APLA2.COSY and TOCSY are required to clarify spin systems,but many spin systems are degenerate and may be distinguished from others with the new selective irradation method. Most of the ~1H chemical shifts have been assighed by the 2D Inverse‘J’ Spectrum experiment and the mean chemical shift caculation and the simulation of the chemical shift values.更多还原