【作者】 杨惠娟； 安国勇； 张国增； 苗琛； 周云； 宋纯鹏；

【Author】 YANG Hui-juan, AN Guo-yong, ZHANG Guo-zeng, MIAO Chen, ZHOU Yun, SONG Chun-peng~* (College of Life Science, He’nan University, Kaifeng, He’nan 475001, China)

【机构】 河南大学生命科学学院；

【摘要】 以拟南芥（Arabidopsis thaliana Columbia）根为材料,利用膜片钳技术测定其根细胞原生质体质膜内向K⁺电流,并对Na⁺对其K⁺电流的影响进行了初步研究,发现Na⁺可明显抑制拟南芥根细胞原生质体的内向K⁺电流,外施Ca²⁺可缓解Na⁺对内向K⁺电流的抑制。说明Ca²⁺参与了质膜上K⁺通道对K⁺/Na⁺的选择性吸收的调节,从而使植物适应盐胁迫。更多还原

【Abstract】 Using patch-clamp techniques, we examined the K⁺ channel activity in the plasma membrane of root cells from Arabidopsis thaliana （ecotype Columbia） after NaCl treatment. Inward currents in the root cells were significantly decreased within 5 min after 50 mmol/L NaCl was perfused to the bath solution. However, addition of 5 mmol/L Ca²⁺ recovered the decrease of inward-rectifying current. These suggest that Ca²⁺ is involved in the regulation of selective uptake of Na⁺/K⁺ through K⁺ channel in root cells under higher Na⁺ concentration, leading to adaptation of plants to salt stress.更多还原