【作者】 陈晓宇； 李青旺； 夏凡； 龙玲； 赵红卫； 韩增胜； 张德福；

【Author】 CHEN Xiao-yu , LI Qing-wang, XIA Fan, LONG Ling, ZHAO Hong-wei , HAN Zeng-sheng , ZHANG De-fu(1. College of Animal Science and Technology, Noth-west Sci-tech University of Agriculture and Forestry, Yangling, Shaanxi 712100, China;2. Laboratory of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agro-genetics and Breeding ,Shanghai 201106, China)

【机构】 西北农林科技大学动物科技学院； 上海市农业遗传育种重点实验室；

【摘要】 本实验就PMSG、hCG、E₂及rpGH对猪卵母细胞体外成熟的影响进行了研究,并比较了BSA、NCS、FCS和L-cysteine在成熟中的作用。结果表明,hCG和PMSG联合使用的促成熟作用显著高于PMSG单独使用（31.9%、17.5%,P<0.05）;E₂在成熟中的作用不显著（35.0%、31.9%,P>0.05）。成熟中NCS的促成熟效果与FCS无显著差异（P>.05）,但两者均显著高于BSA和BSA+FSC（38.4%、30.0%、20.0%、27.3%,P<0.05）。添加6mg/mL-cys和10μg/L rpGH后,体外成熟率为30.4%和50.8%,显著高于其它浓度组（P<0.05,P<0.01）。实验表明,在猪卵母细胞IVM中添加10IU/L hCG、PMSG,0.6mmol/L L-cysteine和10μg/L rpGH有助于卵母细胞成熟;同时添加10%FCS或10%NSC比较适宜。更多还原

【Abstract】 The effects of PMSG, hCG, E2, rpGH, BSA, NCS, PCS and L-cysteine on the IVM of porcine oocytes were studied in this paper. The results demonstrated the rate of matured oocytes with mixture of hCG and PMSG(31.9%)was significantly higher than that of PMSG (17.5%)(P<0.05). Supplemented NSC, PCS, BSA and mixture of BSA and PCS to the IVM system, the rates were 38.4% , 30.0% , 20.0% and 273% respectively. Both NSC and PCS were significantly higher than others(P<0.05). The rates of IVM supplemented with 6 mg/mLL-cys (30.4%) or 10 μg/L rpGH(50.8%) were significantly higher than others. The study indicated the IVM system added 10IU/L hCG, 10IU/L PMSG, 0.6 mmol/L L-cysteine, 10 u g/L rpGH , 10% PCS or 10% NSC was useful to the mature of porcine oocytes in vitro.更多还原