【作者】 王立新； 白俊杰； 陈宏； 罗建仁； 叶星； 简清； 劳海华；

【Author】 WANG Li-xin, BAI Jun-jie, CHEN Hong, LUO Jian-ren, YE Xing,JIAN qing, LAO Hai-hua,(College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China; Pearl River Fisheries Research Institute, Key Laboratory of Tropi & Subtropical Fish Breeding & Cultivation, Chinese Academy of Fishery Sciences, Guangzhou 510380; Institute of Biotechnology, Xuzhou Normal University, Xuzhou, Jiangsu 22116,China)

【机构】 西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室； 中国水产科学研究院珠江水产研究所中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室；

【摘要】 从受精后22h的斑马鱼胚胎中提取总RNA,采用RT—PCR克隆斑马鱼MyoD基因,获得长度约1346bp的目的片段,测序结果表明,该片段含有斑马鱼MyoD基因完整的3’末端,距5’端的起始密码子还有78个碱基,与基因库中已登录的斑马鱼MyoD基因全序列相比,核苷酸序列同源性为98.2%,相对应的编码框所编码的氨基酸序列同源性为98.8%,二者存在的变异包括5次转换,1次颠换,2次缺失和一个微卫星位点多态性。更多还原

【Abstract】 Total RNA was isolated from thirty embryos at 22 h postfertilization in Zebraflsh. The encoding the MyoD gene 3’region was amplified of Zebraflsh by reverse-transcription polymerase chain reaction. The a length of obtained DNA fragment was approximate 1346 bp. The result showed that it was Zebraflsh MyoD gene sequence. Comparing this DNA fragment with the MyoD gene sequence of Zebraflsh recorded in the Gene-bank, the identity of nucleotide sequence was 98.2%, and the identity of amino acid sequence was 98.8%. there were five transitions, two deletions, one tranversion and one polymorphism of microsatellite site between two sequences.更多还原