【作者】 欧阳叙向； 黄生强； 施启顺； 柳小春；

【Author】 OUYANG Xu-xiang, HUANG Sheng-qiang, SHI Qi-shun, LIU Xiao-chun(College of Animal Science and Technology, Hunan Agriculture University, Changsha, Hunan 410128, China; Hunan Biological and Electromechanical Polytechnic, Changsha, Hunan 410127, China )

【机构】 湖南农业大学动物科技学院；

【摘要】 利用比较基因组学的方法,根据绵羊FSH受体基因序列设计引物,以湖南湘东黑山羊基因组为膜板,PCR扩增了FSH受体基因5’端侧翼调控区,通过克隆进行了序列测定及序列分析,并与牛、绵羊该区段序列进行了比较。结果表明:PCR扩增获得的片段为844bp,与中国西门塔尔牛该段序列相比,同源性为94.08%,碱基变异率为5.92%,在—364bp处的左翼发生了多碱基插入,湘东黑山羊该序列有5个碱基的插入,另在-625～-619bp处的7个碱基缺失以及在-630bp处有1个碱基缺失。而与澳洲绵羊的FSHR基因比较,同源性为93.60%,碱基变异率为6.40%,湘东黑山羊该段序列在-145bp和-97bp处各有1个碱基缺失。更多还原

【Abstract】 5’-flanking region and of FSHR gene in Chinese Xiangdong Black goat were amplified by PCR technique, cloned and sequenced. The results of sequence comparison showed as follows: the PCR products was 844 bp .the sequence homology in 5’-flanking region was 94.08% between Chinese Xiangdong black goat and Chinese Simmental bovine, the base-variability was 5.92% accordingly, and was 93.60% between Chinese Xiangdong Black goat and Australian sheep, the base-variability was 6.40% accordingly.更多还原