【作者】 蓝贤勇； 陈宏； 潘传英； 张永德； 张润锋； 田燚；

【Author】 LAN Xian-yong, CHEN Hong, PAN Chuan-ying, ZHANG Yong-de,ZHANG Run-feng, Tian Yi(College of Animal Science and Technology, Northwest Sc- Tech University of Agriculture and Forestry, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China; Institute of Biotechnology, Xuzhou Normal University, Xuzhou, Jiangsu 221116, China)

【机构】 西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室；

【摘要】 本研究首次从西农萨能奶山羊中克隆了IGFBP3基因,并测了序。经序列分析,结果表明:IGFBP3基因序列含有153A,185C,174G,143T,(G+C)%为54.81%,(A+T)%为45.19%;经Oligo6软件分析,该序列含有7个HaeⅢ酶切位点,有2个Alw26I酶切位点和1个TaqI酶切位点。从山羊、绵羊、黄牛和水牛序列对比结果的高度同源性(两两同源性至少为92%)分析,IGFBP3基因相当保守。更多还原

【Abstract】 In this study, IGFBP3 gene in Xinong Saanen dairy goat was cloned and sequenced in first time. The results showed that sequence of IGFBP3 gene had 153 A, 185 C, 174 G and 143 T. This implied that percent contents of (G+C) and (A+T) were 4.81% and 45.19%. In this sequence, there were 7 endonuclease HaeⅢ sites, two endonuclease Alw26l sites and 1 endonuclease Taq\ site after scanning with Oligo 6.0 software. The sequence was high homologous for more than 92% according to comparison of every two IGFBP3 gene sequences among goat, sheep, bovine and water buffalo. It could be safely concluded that IGFBP3 gene was strong conservative.更多还原