【作者】 赖呈纯； 赖钟雄； 桑庆亮； 韩牙琴；

【Author】 Lai Chengchun;Lai Zhongxiong;Sang Qingliang;Han Yaqin Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University Fuzhou 350002

【机构】 福建农林大学园艺植物生物工程研究所；

【摘要】 以天弓石斛的茎段为外植体研究天弓石斛离体培养快繁技术,试验结果表明,附加2.0mg/L BA和0.2mg/L NAA的MS培养基可作为天弓石斛离体培养的起始培养基诱导不定芽;而附加0.2mg/L NAA和4.0mg/L BA的MS培养基适合作为天弓石斛不定芽增殖的培养基,增殖系数可达到5.40;附加3.0mg/L BA、0.2mg/L NAA和100g/L香蕉汁的MS培养基或者附加4.0mg/L BA、0.2mg/L NAA和100g/L香蕉汁的MS培养基都是适合诱导天弓石斛茎段或茎尖产生原球茎或原球茎增殖的培养基。更多还原

【Abstract】 The micropropation of D. pierardii was developed from the explants of youngstem nodes. The young stem nodes were cultured on the solid MS medium with 2.0 mg/L BAand 0.2 mg/L NAA for the inducement of shoots. The results showed that the solid MS mediumwith 0.2 mg/L NAA and 4.0 mg/L BA was suitable for multiplication of shoots and the ratio ofproliferation was 5.40. The solid MS medium with 3.0 mg/L BA, 0.2 mg/LNAA and 100 g/Lbanana extraction or with 4.0 mg/L BA, 0.2 mg/L NAA and 100 g/L banana extraction adapt tothe formation and multiplication of protocorm.更多还原