【作者】 傅玉兰； 谷凤； 杨海燕； 戴英俊； 黄新；

【Author】 Fu Yulan;Gu Feng;Yang Haiyan;Dai Yingjun;Huang Xin Anhui Agricultural University Hefei

【机构】 安徽农业大学；

【摘要】 洋桔梗试管苗的生长受各种因素影响,如温度、湿度、光照、培养基成分、植物激素等,为了批量生产洋桔梗高质种苗,本文围绕着植物激素对试管苗生长、增殖及生根的影响、以及基质成分对试管苗出瓶移栽成活率的影响等进行了研究。研究表明,赤霉素GA3对试管苗茎的伸长生长有明显的促进作用,且以0.8mg/L的效果最好。MS+0.1mg/LNAA+0.2mg/LIAA+0.8mg/LGA₃的激素组合对促进试管苗高生长的效果最佳;6-BA对试管苗茎的伸长有抑制作用,而对新芽增殖有促进作用。对试管苗增殖促进作用最突出的培养基配方为MS+0.5mg/L BA+0.1mg/L NAA+0.2mg/L IAA+0.8mg/L GA₃;而对试管苗生根效果最佳的生长素配方为1/2MS+1.0mg/L IBA。其生根率最高,且生根的时间较短;用炉渣友与珍珠岩按1:1的混合基质,对试管苗移栽成活率效果较突出,成活率达85.7%。更多还原

【Abstract】 The Vitreous plantlets’ growing of Eustoma grandiflorun is affected by allkinds of factors, such as temperature, humidity, light, the elements of culture medium. In orderto product numbers of Eusments grandiflorun, we studied the affection about plants incretion tothe growth of the vitreous plantlets, the elements of culture medium to the survival ration oftransplanting out of curette. The results show the combination of incretion MS+0.1 mg/LNAA+0.2 mg/L IAA+0.8 mg/LGA₃make vitreous plantlets grow best, MS+0.5 mg/L BA+0.1mg/L NAA+0.2 mg/L IAA+0.8 mg/L GA₃ is the best dispensition of faciliting prociferation,1/2MS+1.0 mg/L IBA is the best dispensition of rhizogensis, and have shorter time of rootage;mixed substrate of slag pleasure and pearlite 1:1 can make the rate of survive after transplantinghigh, reaching to 87.5%更多还原