【作者】 张妙霞； 赖钟雄； 黄玉吉； 吴金寿；

【Author】 Zhang Miaoxia;Lai Zhongxiong;Huang Yuji;Wu Jinshou Institute of Subtropical Fruits, Fujian Agric. and Forestry Univ, Fuzhou 350002

【机构】 福建农林大学亚热带果树研究所；

【摘要】 以天宝蕉吸芽茎尖培养产生的分生芽为外植体,通过薄层培养和愈伤组织的诱导,为香蕉的转基因再生系统研究提供材料。研究发现横切薄层切片的芽再生率高于纵切,而愈伤组织诱导的最佳培养基配方为MS+2,4-D₆+KT_0.5+AgNO₃5mg/L+Su3%+Ag0.9%。香蕉芽增殖的最佳培养条件为30℃黑暗培养。更多还原

【Abstract】 A protocol was developed using banana (Musa spp.) shoot tip as explants,through thin-cell-layer culture and callus induction to produce the maximum numbers of shoots,which was the material for banana transformation and regeneration. It shows that the shootsregeneration frequency of transverse thin-cell-layer culture was higher than longitudinalthin-cell-layer culture. The best medium for callus induction was MS-based mediumsupplemented with 6mg/1 2,4-D, 0.5mg/1 KT, 3% sugar and 0.9% agar. The best condition forbanana multiplication was cultured in dark at 30℃.更多还原