【作者】 吴金寿； 赖钟雄； 陈义挺； 林珊珊； 叶芝萍；

【Author】 Wu Jinshou;Lai Zhongxiong;Chen Yiting;Lin Shanshan;Ye Zhiping Institute of Horticultural Biotechnology; Fujian Agriculture and Forestry University Fuzhou, Fujian 350002 Fuijan Academy of Agricultural Sciences. Fujian 350003

【机构】 福建农林大学园艺植物生物工程研究所；

【摘要】 以芳樟嫩茎为外植体,进行了离体培养快繁技术的研究;试验结果表明:在改良MS+2mg/L 6-BA+0.1mg/LNAA固体培养基中诱导不定芽,诱导率高;用改良MS+2mg/L 6-BA+0.1mg/LIBA+0.2mg/LGA₃和无GA₃的固体培养基交替培养可大量增殖,繁殖系数高达10～20倍;芽苗在1/2MS+0.8mg/LIBA+0.2mg/LNAA+2g/L蔗糖的固体培养基中,诱发生根,壮苗最好。更多还原

【Abstract】 The technique of in vitro culture and rapid propagation of seedling productionof Cinnamomum camphora var. linaloolifera Fujita was developed from the explats takenfrom delicate caudex. The results showed that the inducing coefficient was the highest on thesolid MS medium supplemented with 2 mg/L BA and 0.1mg/L NAA for inducing thegermination adventitious buds. The improved solid MS medium with 2 mg/L BA, 0.1 mg/LNAA, 0.2 mg/LGA₃ （or without GA₃） were alternately used for multiplication, theproliferating rate was over 10-20times, plantlets rooting and growth was best on the solidmedium supplemented with 1/2MS+0.8mg/LIBA+0.2mg/LNAA+2g/L sugar.更多还原