【作者】 徐琼芳； 陈孝； 田芳； 李连城； 杜丽璞； 马有志； 辛志勇；

【Author】 XU Qiong-fang;CHEN Xiao;TIAN Fang;LI Lian-cheng;DU Li-pu;MA You-zhi;XIN Zhi-yong Institute of Crop Breeding and Cultivation, CAAS, 100081

【机构】 中国农业科学院作物科学研究所农业部重点开放实验室；

【摘要】 以小麦品种京411作为受体材料,取其幼胚进行愈伤诱导,通过基因枪将抗蚜虫的雪花莲凝集素(Galanthus nivalis agglutinin,GNA)基因转入小麦愈伤组织,每代进行PCR检测和接麦蚜实验证明获得了T5代较稳定的转基因品系6个。并以转GNA京411为抗蚜虫亲本与优质小麦杂交获得了优质的、抗蚜虫小麦新品系。更多还原

【Abstract】 The immature embryos of wheat plants Jing 411 were taken to be culturedfor callus induction. The plasmid containing GNA (Galanthus nivalis agglutinin) gene wastransferred to the embryogenic callus by using particle bombardment. The regeneratedtransgenic wheat plants and the successive generations were tested for PCR analysis andaphids resistance analysis. Finally,6 GNA gene stably inherited transgenic wheat lines inT5 generation were identified. We also generated high--quality and aphids--resistant wheatlines by crossing the transgenic Jing411 wheat lines with high--quality wheat lines.更多还原