【作者】 张新春； 李自超； 庄炳昌； 张承亮； 王国英； 付永彩；

【Author】 Zhang Xinchun;Li Zichao;Zhuang Bingchang Zhang Chengliang;Wang Guoying;FU Yongcai Key Lab of Crop Genomics and Genetic Improvement, Ministry of Agriculture; Key Lab of Crop Genetic Improvement, Beijing, China Agricultural University, Beijing, 100094, P. R. Jilin Academy of Agricultural Sciences, Jilin Province 136100 College of Biological Sciences, China Agricultural University, Beijing 100094

【机构】 中国农业大学农业部作物基因组学与遗传改良重点实验室和北京市作物遗传改良重点实验室； 吉林省农业科学院； 中国农业大学生物学院；

【摘要】 利用基因枪转化技术将来源于大肠杆菌的mtlD（1-磷酸甘露醇脱氢酶）基因导入水稻。转基因植株的PCR扩增、Dot blotting和Northern blotting检测表明:mtlD基因已整合到水稻基因组中,并在T₁代转基因植株中稳定表达。T₁代转基因植株获得了0.75%NaCl抗性。转基因水稻的叶片相对电导率和地上地下部Na⁺、K⁺含量测定结果表明:与非转化水稻相比,Na⁺向地上部运输受抑,盐分对质膜的伤害减轻,进一步说明mtlD基因在水稻中的表达提高了水稻的耐盐性。遗传检测采用1%NaCl和50mg/L潮霉素B筛选,T₁代转基因水稻耐盐和盐敏感分离比例以及抗潮霉素B和潮霉素B敏感分离比例均大于3:1。更多还原

【Abstract】 Escherichia coli mt1D （Marinitol-1-phosphate dehydrogenase） gene drivenby the Actinl promoter was successfully introduced into lowland rice （Oryza sativa L.ssp. indica） by microprojectile bombardments. Analyses of PCR, dot blotting, andNorthern blotting show that mtlD gene was integrated into lowland rice genome andexpressed normally in transgenic plants. Growth of some transgenic plants were superiorto controls obviously in salinized pots （0. 75% NaCl）. Results of relative electronicconductivity and contents of K⁺ and Na⁺ demonstrate that transgenic plants had lessmembrane damage and lower Na⁺ to K⁺ ratio of transgenic plants than controls under saltstress. Ratios of salt-tolerant to salt-sensitive plants and hygromycin--resistant tohygromycin--sensitive plants of T₁ progeny were larger than 3: 1.更多还原