【作者】 叶兴国； 黄益洪； 徐惠君； 杜丽璞； 吴成军； 周淼平； 陆维忠；

【Author】 Xingguo Ye;Yihun Huang;Huijun Xu;Lipu Du;Cheng jun Wu;Miaopin Zhou;Weizhong Lu Institute of Crop Breeding and Cultivation, CAAS, Beijing, 100081 Institute of Genetics and Physiology, JSAAS, Nanjing, 210014

【机构】 中国农业科学院作物育种栽培研究所； 江苏农业科学院遗传生理研究所；

【摘要】 利用农杆菌介导法将BCL和RIP二个与细胞凋亡有关的基因分别导入了小麦品种扬麦10号和Bobwhite,转化效率分别为1.60%和1.25%。Southern检测结果表明,细胞凋亡基因已稳定整合到小麦染色体上,多数植株为单拷贝整合。Northern检测结果表明,细胞凋亡基因能够在小麦遗传背景高水平转录为RNA。转基因植株T1代分离比例为2.11～2.33:1,表明了外源基因在后代中的稳定遗传。BCL转基因植株对赤霉病表现出一定抗性,可望培育抗小麦赤霉病的新材料。更多还原

【Abstract】 BCL and RIP genes related to cell apodosis were transferred into Yangmai10 and Bobwhite, Triticum aestivum CV., by Agrobacterium tumefaciens with theefficiencies of 1.60% and 1.25%, respectively. The transgenic plants were confirmed bynptII ELISA first, then by Southern blot and Northern blot. The segregation ratio of thegenes in T1 generation was 2.11～2.33:1. The results indicated that the alien genes hadintegrated into wheat chromosomes, transcripted into RNA, and inherited from generationto generation. By artificial inoculation of pathogen, the transgenic plants showed someresistance to scab in greenhouse.更多还原