【作者】 徐惠君； 杜丽璞； 张新梅； 庞俊兰； 马有志； 叶兴国； 程顺和； 吴宏亚； 陈炯； 陈剑平；

【Author】 Xu Hui-Jun;Du Li-Pu;Zhang Xinmei;Pang Jun-Lan;Ma Youzhi;Ye Xing-Guo;Chen Shun-He;Wu Hong-Ya;Chen Jiong;Chen Jianping Ministry Key Lab for Crop Genetics and Breeding, Chinese Academy of Agricultural Sciences, Beijing 100081, China Lixiahe Agricultural Institute of Jiangsu Province, Yangzhou 225002 China Virology Laboratory of Zhe jiang Academy of Agricultural Sciences, Hangzhou 310021 China

【机构】 中国农业科学院作物育种栽培研究所农业部作物遗传育种重点实验室； 江苏省里下河地区农科所； 浙江省农业科学院植物病毒实验室；

【摘要】 筛选出适宜小麦转化的优良基因型扬麦158和扬麦10号。以扬麦158的胚愈伤组织为受体,利用基因枪共转化法将小麦黄花叶病毒复制酶基因（WYMV-Nib8）转入了小麦。T₀代经PCR扩增分析,获得了14株含WYMV-Nib8基因的转化植株,T₁、T₂代跟踪分子检测和T₂、T₃代田间抗性鉴定结果表明,获得了4个抗WYMV的转基因株系,同时利用PCR技术筛选到只含功能基因而没有标记基因的扬麦158转基因小麦。更多还原

【Abstract】 Two wheat genotypes with high tissue culture ability, Yangmai 158 andYangmai 10, were screened from ten commercial varieties for the followingtransformation. Then, the seven days immature embryo calli of Yangmai 158 used asreceptor of Nib8 target gene, which was isolated from wheat yellow mosaic virus, werebombardmented by biolistic particle. A lot of resistant plantlets to 3∽5 mg/l Biolophoswere achieved on the selection medium. By PCR analysis in the T₀ generation, fourteen transgenic plants were identified. Four transgenic wheat lines resistant to the diseasestrongly were obtained finally combining the molecular test of T₁, T₂ and WYMV field testof T₂.,T₃ Excision of bar gene from transgenic wheat through the methods of PCR analysisand shoes the transgenic wheat which only have target gene without selectable markergene.更多还原