【作者】 向太和； 杨剑波； 李莉； 王永杰； 黄大年；

【Author】 XIANG Tai-he;YANG Jian-bo;LI Li;Wang Yong-jie;HUANG Da-nian Rice Research Institute at Anhui Academy of Agricultural Sciences, Key Laboratory of Rice Genetics Breeding of the National Agriculture Ministry, Hefei 230031 China National Rice Institute, Hangzhou 310006

【机构】 安徽省农业科学院水稻研究所农业部水稻遗传育种重点开放实验室； 中国水稻研究所；

【摘要】 水稻苯达松敏感致死基因(ben)在杂交水稻制种生产中具有广阔的应用前景。本研究根据中国水稻全基因组框架序列中的跨叠克隆Contig10968,成功地将与Ben/ben共分离的RAPD标记OPG18/972和OPG18/943步移延伸至8138bp和8103bp。利用生物信息学技术,通过电子杂交将OPG18/972和OPG18/943及步移后的序列定位于第2染色体150.5cM处,进一步将Ben/ben初步定位于第2染色体150.5cM处。通过ORF、启动子及基因预测软件分析,Ben可能是编码某种受体蛋白的基因或者催化6-OH-苯达松与葡萄糖缀合、类似淀粉合成酶的基因,而ben可能是Ben的调控因子如启动子、内含子等突变而致。更多还原

【Abstract】 The PCR primer sets were designed according to contig 10968 from a draftsequence of the rice genome(Oryza sativa L ssp. indica). The RAPD markers OPG18/972and OPG18/943 cosegragatied with Ben/ben genes were successfully walked to 8138bp and8103bp. OPG18/972 and OPG18/943 locus, and Ben/ben were anchored to chromosome 2at 150cM in silico. Open reading frame, promoter and Ben/ben were predicted. Theresults give a clue that Ben gene maybe code a receptor protein or code a certain starchsynthase--like which metabolizes 6--hydroxybentazon (6--OH--bentazon) to the glucoseconjugate. The change of Ben gene at regulatory elements (such as promoter, intron etc)perhaps produced the ben gene.更多还原