节点文献
辣椒不同外植体离体培养再生
In Vitro Plant Regeneration from Cotyledon Explants and Hypocotyl Explants of Pepper
【作者】 董兆龙； 唐亮； 刘文轩； 邓志瑞； 郭志华； 陈沁；
【Author】 DONG Zhao-long;TANG Liang;GUO Zhi-hua;LIU Wen-xuan;Deng Zhi-rui;CHEN Qin School of Life Science, Shanghai University, Shanghai 200436, China
【机构】 上海大学生命科学学院；
【摘要】 8个品种的辣椒子叶和下胚轴接种在附加不同植物激素的MS培养基上,经过芽诱导、芽伸长、生根、移栽入盆四个阶段,得到完整的再生植株。实验比较了苗龄、不同的外植体类型对分化率的影响,结果发现8～10天的苗是最佳的,子叶的分化率高于下胚轴。培养基中添加AgNO3明显促进外植体的分化,GA3是芽伸长的关键因子,IBA的生根效果要好于无激素MS培养基。通过这一过程,建立起一个基于最佳激素组合的高效植株再生体系。
【Abstract】 Regenerated plants were obtained from cotyledon and hypocotyl explants ofeight pepper varieties(Capsicum annuum L.)cultured on basal MS medium supplementedwith different kinds and different concentrations of plant hormone by four--step cultureprocedure including shoot induction, shoot elongation, rooting of excised shoot andtransplanting into soil. The effect of seedling stage on frequency of shoot induction wasobserved with a result that seedlings of 8～10 days old were best for shoot induction. Ingeneral, the frequency of shoot induction from cotyledon explants was higher than thatfrom hypocotyl explants in terms of explant types. AgNO3 significantly increased thefrequency of shoot induction from all varieties. GA3 was the key factor in shootelongation. The elongated shoots cultured on MS basal medium were easier to root thanthat on MS basal medium with IBA. Through above four--step procedure, a genetictransformation system has been established.
【Key words】 Capsicum annuum L.； in vitro plant regeneration； cotyledon； hypocotyl；
- 【会议录名称】 全国作物细胞工程与分子技术育种学术研讨会论文集
- 【会议名称】全国作物细胞工程与分子技术育种学术研讨会
- 【会议时间】2003-03
- 【会议地点】中国江苏苏州
- 【分类号】S641.3
- 【主办单位】中国农业生物技术学会