【作者】 刘艳芝； 李俊波； 王玉民； 王中伟； 纪锋； 董英山；

【Author】 Liu Yanzhi;Li Junbo;Wang Yumin;Wang Zhongwei;Ji Feng;Dong Yingshan Jilin Province Key Open Lab. On Agro-biotechnology, Jilin Academy of Agricultural Sciences, Gongzhuling 136100

【机构】 吉林省农科院吉林省农业生物技术重点开放实验室；

【摘要】 采用水稻主栽品种的成熟胚和幼穗诱导胚性愈伤组织,干燥处理提高了胚性愈伤组织分化率,建立起水稻转基因的高效受体系统。此系统培养基简单,培养周期短,适宜转基因操作,严格挑取胚性愈伤进行继代培养是关键技术。更多还原

【Abstract】 Calli were induced from immature embryos and young spikes of differentcommercial rice varieties and embryonic calli were selected and subcultured. Thedifferentiation rate embryonic calli of was promoted by dry treatment. This system,featured with simple media and short culture period, is highly efficient and can be used forrice genetic transformation. The key technique is the selection of embryonic calli forsubculture.更多还原