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马蔺天然群体遗传多样性的AFLP分析

Genetic Diversity of Iris lactea Determined by AFLP Marker

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【作者】 牟少华孙启祥彭镇华孙振元岳永德

【Author】 Mu Shaohua Sun Qixiang Peng Zhenhua Sun Zhenyuan Yue Yongde International Center for Bamboo and Rattan, Beijing, 100102;Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091

【机构】 国际竹藤网络中心中国林业科学研究院林业研究所

【摘要】 利用AFLP技术,选择EcoR Ⅰ/Mes Ⅰ这一酶切组合,应用18对EcoR Ⅰ+3/Mes Ⅰ+3引物组合进行选择性扩增,检测了10个马蔺种群的基因组DNA多态性。共扩增出1164个遗传位点,其中 752个多态位点,多态率为65.11%。并通过Jaccard的方法将电泳带矩阵转化为遗传相似性系数矩阵, 进行了UPGMA聚类分析。结果表明:当取阈值为0.74时,可以将10个种群分为四大类群。第一类, 长春马蔺与其它种源的亲缘关系较远,单独聚为一类:宁夏固原、甘肃民勤、武威、北京、山东泰山五个种群亲缘关系较近,聚为一类;内蒙古太仆寺和西乌旗、新疆乌鲁木齐聚为一类;河北涿洲马蔺种群单独聚为一类。马蔺群体间的亲缘关系远近与其所处的地理位置有很大的关系,尤其与纬度因子的关系十分密切。

【Abstract】 The AFLP (Amplified Fragment Length Polymorphism) technique was used to analyze genetic diversity of 10 different populations of Iris lactea collected from China. Genomic DNA was digested with EcoR Ⅰ and Mse Ⅰ enzymes and amplified with 18 EcoR. Ⅰ+3 / Mes Ⅰ+3 primer combinations. AFLP analysis produced 1 164 scoreable bands, of which 752 (65.11%) were polymorphic. Cluster analysis was based on the UPGMA method. Populations in Changchun and Zhuozhou city showed the greater genetic variability and were separately classified into two groups. Populations in Guyuan, Minqin, Wuwei, Beijing and Tais-han were classified into one group, and those in Taipu, Xiwuqi and Wulumuqi were in another group. The genetic relationships among the populations were related to their locations, especially to the latitude.

【关键词】 马蔺(Iris lactea)AFLP遗传多样性多态性
【Key words】 Iris lacteaAFLPGenetic diversityPolymorphism
  • 【会议录名称】 第二届热带亚热带植物资源的遗传多样性与基因发掘利用研讨会论文集
  • 【会议名称】第二届热带亚热带植物资源的遗传多样性与基因发掘利用研讨会
  • 【会议时间】2006-11
  • 【会议地点】中国海南三亚
  • 【分类号】S688.4
  • 【主办单位】海南省生物工程协会
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