【作者】 宋成标； 王锐萍； 杜道林； 李迪； 白羊年；

【Author】 Song Chengbiao Wang Ruiping Du Daolin Li Di Bai Yangnian Hainan Institute of Tropical Agricultural Resources, Sanya, 572025; Department of Biology, Hainan Normal College, Haikou, 571158

【机构】 海南省热带农业资源开发利用研究所； 海南师范大学生物系；

【摘要】 图位克隆是在不清楚基因产物结构和功能的情况下,根据基因在染色体上都有稳定的基因座实现的。随着各种分子标记技术和高质量基因组文库构建技术的快速发展,图位克隆现已经成为分离生物体基因的一种常规技术。本文主要概述了图位克隆的一般步骤,包括目的基因的初步定位、精细定位和遗传做图、染色体步行和登陆及利用功能互补实验鉴定目的基因。最后,对图位克隆技术存在的局限和发展前景作了初步的分析。更多还原

【Abstract】 Map-based cloning is conducted when the structure and function of gene products were unclear-ly known and the functional genes have their particular gene loci on chromosomes,. With the rapid development of molecular marker technologies and constructing technologies of high quality genomic library, map-based cloning has already become a general biotechnique for gene isolation. This paper summarized the main processes of the map-based cloning including preliminary mapping, fine scale-mapping of candidate gene, building genetic map, chromosome walking or landing and finally function complement experiment to identify candidate gene. The problems and development trends of map-based cloning are also analyzed in this paper.更多还原