【作者】 郭华春； 杨洁； 沙本才； 肖关丽；

【Author】 GUO Hua-chun, YANG Jie, SHA Ben-cai, XIAO Guan-li (College of Agriculture and Bio-technology,Yunnan Agricultural University, Yunnan Kunming 650201,China)

【机构】 云南农业大学农学与生物技术学院；

【摘要】 近年来云南人工种植灯盏花发展迅速,但种苗生产及供给成了发展的瓶颈,为解决灯盏花种苗快繁问题,对灯盏花的愈伤组织诱导与植株再生进行研究,试验以灯盏花的叶片和叶柄作为外植体,以MS为基本培养基,研究BA和NNA不同浓度配比对愈伤组织诱导和植株再生影响。结果表明:叶柄诱导愈伤组织的最佳培养基为C1培养基(MS+6-BA 1．0 mg／L+NNA 1．0 mg／L),叶片诱导愈伤组织的最佳培养基为C3培养基(MS+6-BA 1．0 mg／L+NNA 5．0 mg／L);来自叶柄的愈伤组织更容易诱导出再生植株,再生植株诱导培养基以B3培养基 (MS+BA 1．0 mg／L+NNA 5．0 mg／L)为好。更多还原

【Abstract】 We carried out the comparative experiment of testing how different culture mediums affect callus inducing and plant’ s regeneration. The result indicated that the best culture medium for callus inducing from leafstalk is C1 (MS+BA 1.0 mg/L+NNA 1.0 mg/L) , the best culture medium for callus inducing from leaf is C3 (MS+BA 1.0 mg/L+NNA 5.0 mg/L) . The callus which is from leafstalk can easily induce new plants. Thus, it is better to choose B3 (MS+BA 1.0 mg/L+NNA 5.0 mg/L) as the callus to induce new plants.更多还原