【作者】 张仲凯； 方琦； 丁铭； 李婷婷； 彭潞波； 张丽珍； 李展； 苏晓霞；

【Author】 ZHANG Zhong-kai FANG Qi DING Ming LI Ting-ting PENG Lu-bo ZHANG Li-zhen LI Zhan SU Xiao-xia(Biotechnology Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650223,China)

【机构】 云南省农业科学院生物技术研究所；

【摘要】 应用超薄切片法制样电子显微镜观察,对番茄斑萎病毒(TSWV)和烟草花叶病毒(TMV)复合侵染的大花蕙兰同一病叶的细胞病理进行了研究。结果表明:无症的叶部其亚细胞结构较完整,在叶绿体旁及细胞质中有少量的 TWV 聚集块;在表现为花叶的部位亚细胞器不同程度病变,TMV 粒体聚集或散布于细胞质中;在表现为黄化的叶部亚细胞大部分崩解,有的细胞中大量聚集或散布 TMV 粒体。有的细胞中则散布或聚集 TSWV 粒体,且在细胞质中出现病毒基质或空泡囊状结构,TSWV 被膜粒体散布或聚集于细胞壁中,有的韧皮部细胞中充满纤维状内含体。更多还原

【Abstract】 Study on the cytopathology of Cymbidinm faberi within complex infection of Tomato spotted wilt virus(TSWV) and Tobacco mosaic virus(TMV),by using ultrathin sectioning observation of electron microscopy,has been carried out. The results indicated:the subeellular structure of symptomless part of diseased leaf was nearly intact,with fewer assemble bodies of TMV particles in the cytoplasma or near the chloroplasts.The subcellular structure of mosaic part of diseased leaf was lesioned different degree,a lots of assemble bodies of TMV particles and single TMV particle distributed in the cyto- plasma.The subcellular structur of yellow part of diseased leaf was avalanche.A assemble of bodies or single particle of TMV distributed in the cytoplasma of some cells.A assemble bodies or single particle of TSWV distributed in the cytoplas- ma of other cells.There were vironplasma and vesicular bodies in the cytoplasma.Enveloped particles of TSWV distributed separately or aggregated in cell walls.Filamentous inclusion distributed in the phloem cells.更多还原