【作者】 王小燕； 蔡继业； 梁志红； 赵涛；

【Author】 WANG Xiao-yan~1 CAI Ji-ye~(1*) LIANG Zhi-hong~2 ZHAO Tao~1 1 Department of Chemistry,Jinan University,Guangzhou Guangdong 510632; 2 Center of Experiment and Technology,Jinan University,Guangzhou Guangdong 510632,China

【机构】 暨南大学生命科学技术学院化学系； 暨南大学实验技术中心；

【摘要】 目的：利用原子力显微镜(AFM)对猪和牛的Ⅰ型胶原蛋白在溶液中的聚集行为进行微观结构的形态学研究。方法：将Ⅰ型胶原蛋白溶液,滴至新鲜剥离的云母片上,空气中干燥后,用原子力显微镜观察。结果：发现来自猪和牛的Ⅰ型胶原蛋白在浓度为32μg/ml 时以单体形式和聚集体形式混合存在,在浓度为4mg/ml 时两种Ⅰ型胶原蛋白自发装配成胶原纤维,形成多孔网状结构,孔隙大小为：牛,100～150nm；猪,170～200nm。孔隙率：牛,575个/ μm2；猪,193个/μm~2。平均粗糙度为：牛,3．1nm；猪,8．7nm。均方根粗糙度为：牛,4．0nm；猪,11．4nm。平均高度：牛, 50．9nm；猪,52．3nm。Rp-v：牛,72．1nm；猪,102．7nm。结论：利用原子力显微镜能够准确直观地观察到Ⅰ型胶原蛋白形成的胶原纤维的种属差异性,AFM 技术是一种理想的胶原纤维微观结构的表征方法。更多还原

【Abstract】 Objective:To study the aggregation behavior of I type collagen from pig and bovine skin by means of atomic force microscopy （AFM）.Methods:I type collagen dispersions with certain concentrations were prepared.After drying in air,they were imaged by AFM. Results:It was found that collagen from different animal’s skin couldn’t exist as monomers.Monomers and aggregates existed simultaneously in the solution even at low concentration.At high concentration,the ultrastructure of different collagen fibril was also different.Five parameters of factor of porosity,R_p-v,R_a,R_rms and Mean Ht were measured.Conclusion:By AFM the difference of collagen fibril from different species can be clearly gained.This AFM technology is an excellent method for the imaging of collagen fibril.更多还原