【作者】 张玉忠； 周百成； 曾呈奎； 刘宁； 庞世瑾；

【Author】 Zhang Yuzhong Zhou Baicheng Zeng Chengkui(C. K. Tseng) Liu Ning~(**) Pang Shijin~(**) (Institute of Oceanology,Chinese Academy of Sciences,Qingdao,266071)(~(**)Beijing Laboratory of Vacuun Physics,Chinese Academy of Sciences,Beijing 100080)

【机构】 山东大学生命科学学院微生物研究所； 中国科学院海洋研究所； 中国科学院北京真空物理实验室；

【摘要】 R-藻红蛋白(R-PE)从海洋红藻多管藻中分离获得,然后用扫描隧道显微镜对其结构进行了研究。结果表明当 R-PE 在基底高定向石墨(HOPG)上吸附时,大部分蛋白分子聚集在一起,少数单个的蛋白分子分布于 HOPG 的表面。进一步缩小扫描范围,可以清楚地观察到 R-PE 分子为圆盘状结构,但分子中央的孔洞不象 C-藻蓝蛋白那样清晰,这是由于γ亚基位于分子的中央孔洞内。更多还原

【Abstract】 R-PE was isolated from marine red alga Polysiphonia urceolata,and STM was used to investigate its topo- graphy.It was shown that most of the R-phycoerythrin molecules assembled together while absorbing on the highly oriented pyrolytic graphite(HOPG)surface;at the meantime,a few of single molecules distributed on HOPG surface.With higher resolution,the topography of the R-PE molecules were observed clearly,which were disc-like in shape.Otherwise,no obvious channel in the centre of the disc like that in C-phycocyanin was seen, which was due to the effect of the γ sununit in the channel.更多还原