【作者】 刘青； 骆斯慧； 陈灵；

【Author】 LIU Qing LUO Si-hui CHEN Ling (Shanghai Jiaotong University,College of Life Science and Technology,Shanghai,200240 China)

【机构】 上海交通大学 生命科学技术学院；

【摘要】 为建立马兜铃酸（Aristolochic acid,AA）肾病大鼠模型以及研究丹酚酸 B（Salvianolic acid B,SalB）对于马兜铃酸的干预作用,本实验分别采用了体内灌胃 SD 大鼠的方法并测定尿肾功能生化指标以及体外小鼠淋巴瘤细胞试验（Mouse Lymphoma Assay,MLA）微孔法.结果表明用5 mg/kg·d 剂量的马兜铃酸灌胃大鼠及用10 mg/kg·d 的丹酚酸 B 治疗所得到的β₂-微球蛋白、尿 N-乙酰-β-D-氨基葡萄糖苷酶、血清肌酐和血清尿素氮生化指标都有相应变化,与理论结果一致;而 MLA 实验结果表明丹酚酸 B 在100μg/mL 和200μg/mL 对于马兜铃酸100μg/mL 均具有干预作用,可减小细胞毒性,降低突变频率,且200μg/mL 丹酚酸 B 效果更好.更多还原

【Abstract】 To establish the rat model of aristolochic acid（AA）nephropathy and study intervention effect of salvianolic acid B（SalB）on aristolochic add,the author used salt-depleted rats fed orally,measured biological markers of renal function, and adopted mouse lymphoma assay microwell method.The result showed that the change of urinary β₂-microglobulin,uri- nary N-acetyl-β-D-glucosaminidase,serum creatinine and BUN of two groups of rats which had been fed orally with AA （5mg/kg·d）and AA（5 mg/kg·d）+SalB（10 mg/kg·d）accorded to academic results;the result of MLA indicated that at the dose of 100 μg/mL and 200μg/mL,salvianolic acid B had intervention effect toward aristolochic acid,it could reduce the cytotoxicity and mutagenic toxicity of aristolochic acid,200μg/mL of salvianolic acid B had better effects.更多还原