【作者】 杨合同； 赵晓燕； 陈凯； 李纪顺； 郭勇； 王贻莲；

【Author】 YANG He-tang,ZHAO Xiao-yan,CHEN Kai,LI Ji-shun,GUO Yong,WANG Yi-lian (Institute of Biology,Shandong Academy of Sciences,Jinan 250014,China; School of Life Science,Shandong University of Technology,Zibo 255049,China; Biotechnology Research Center of Shandong Academy of Sciences,Key Laboratory of Applied Microbiology of Shandong Province,Jinan 250014,China )

【机构】 山东省科学院生物研究所； 山东省科学院中日友好生物技术研究中心山东省应用微生物重点实验室；

【摘要】 从山东省棉田自然死亡的棉铃虫上分离到常见致病菌至少有8种,经鉴定为:苏云金杆菌(Bacillus thuringiensis)、蜡状芽孢杆菌(Bazillus cereus)、铜绿假单胞菌(Pseudomonas aeruginosa)、球孢白僵菌(Beauveria bassiana)、卵孢白僵菌(B．tenella)、青霉菌属真菌(Penicillium spp．)、拟青霉属真菌(Paecilimrces spp．)和蛾霉属真菌(Nomurae spp．);得到一株对棉铃虫高毒的苏云金杆菌菌株Hu,其毒力比普遍使用的Bt菌系7216高,二者的LC50分别为15．1μg/mL和26．28μg／mL。蛾霉属真菌对棉铃虫有明显的拒食作用和杀伤作用,但人工培养时生长十分缓慢。更多还原

【Abstract】 Eight species of pathogenic microbes isolated from larvaes of Helicoverpa armigera naturally diseased and collected from cotton fields in Shandong Province were identified. They were Bacillus thuringiensis, Bacillus cereus, Pseudomonas aeruginosa, Beauveria bassiana, Beaiweria tenella , Penicillium spp. , Paecilimyces spp. and Nomurae spp. Among them, a Bacillus thuringiensis isolate numbered Hu was the most virulent to Helicoverpa armigera based on bioassay. Toxicity of Hu to H. armigera lavae was higher than that of Bt7216 which was commonly used in biopesticide plants, with LC50 of Hu and Bt7216 were 15.1μg/mL and 26.28μg/mL respectively. Nomurae spp. had an antifeedant effect and was pathogenic to H. armigera, but was slow in growth on PDA.更多还原