【作者】 赵渤年； 刘青； 丁晓彦；

【机构】 山东省中医药研究院；

【摘要】 目的:采用,PLC法测定九味羌活颗粒中欧前胡素的含量。方法:以C18为固定相,乙腈-水 (55:45,V/V)为流动相,检测波长为250nm。结果:线性范围0．18-1．2μg,平均回收串为99．79％,RSD 为1．22％。结论:该方法简便可行,可作为九味羌活颗粒中欧前胡素的含量测定方法。更多还原

【Abstract】 Objective:The essay studied granule preparation of Jiuweiqianghuo Granule and established an HPLC method for determination of Imperatonn and Isoimperatonn in Jiuweiqianghuo Granule.Method:When disposed of sample , the solvent varieties, polarities and extraction methods were systematically studied. At last, acetoacetate was choosed as the better solvent . because of the more way to extract Imperatorin and Isoimperatonn from Jiuweiqianghuo Granule .The sample was extracted by ultrasonic wave in acetoacetate , standed and filtered, evaporated to dryness on a water bath (65-70 ℃).Then methnol made it to 10ml.The HPLC method was carried out on C18 (HanBang 200nm×4.6nm, 5μm)column with acetonitrile-water solution as a mobile phase .The flow rate was 1.00ml/min.The detection wavelength was at 250nm and the column temperature was 30 ℃ .Reasuh:The standard cure was linear within the concentration range of 0.180-1.20μg Isoimpentorin and of 0.120-1.200μg Imperatorin. which indicated the method can be used to the determination of Imperatorin and Isoimperatonn from Jiuweiqianghuo Granule and the formulation of Jiuweiqianghuo Granuie.Conclusion:The established method was simple, workable , accurate, rapid and reproducible Jt could be used for quality control for Jiuweiqianghuo Granule as a commodity.更多还原