【作者】 陈锋； 郭峰； 田纪春； 孟庆伟； 赵世杰；

【Author】 CHEN Feng TIAN Ji-chun MENG Qing-wei ZHAO Shi-jie (College of Life Science; College of Agronomy, Shandong Agricultural University; Key Laboratory of Crop Biology of Shandong, Tai’an, 271018, Shandong, China)

【机构】 山东农业大学生命科学学院； 山东农业大学农学院；

【摘要】 以叶绿素快相荧光动力学曲线(OJIP)为探针,探讨了高产小麦品系01-35在灌浆后期高温胁迫对光系统Ⅱ(PSⅡ)功能的影响。结果表明,在37℃～43℃范围内,随温度升高QA还原程度和还原速率增大,至43℃时分别比室温下增大了23．89％和24．09％,这表明QA→QB电子传递受到抑制; 43℃时PSⅡ的电子受体库降至室温下的47．4％,表明高温胁迫伤害到了PSⅡ受体库:而PSⅡ供体侧未受影响。当温度达到46℃时,QA还原程度和还原速率分别比室温下增大了13．95％和20．48％, 但比43℃时显著下降,而PSⅡ的电子受体库与43℃时相比无显著变化,表明46℃时PS II供体侧受到伤害。与对照品种鲁麦14相比,高温胁迫下高产小麦的捕光色素复合体仍能捕获更多的光能,而且将捕获的光能更多的用于电子传递,表明高产小麦的捕光色素复合体以及电子传递体耐受高温的能力较强,最终能够维持较高的电子传递能力。更多还原

【Abstract】 Effects of short-term high temperature stress on photosystem II (PS II) functions were investigated in high yield winter wheat cultivar 01-35 at late grain filling stage using fast O-J-I-P chlorophyll a (Chl a) fluorescence transients. The results showed that with stress temperature elevated from 37 ℃ to 43 ℃, the extent and velocity of QA reduction were enhanced gradually, which were 23.89% and 24.09% higher at 43 ℃ than those at room temperature, respectively. This suggested that the electron transport from QA to QB was inhibited. The electron acceptor pool size of PS II that included QA, QB and PQ at 43 ℃ decreased to 47.4% of that at room temperature, indicating that the electron acceptor pool of PSII was damaged. However, the electron donor side of PS II was not damaged from 37 ℃ to 43 ℃. When temperature increased to 46 ℃, the extent and velocity of QA reduction were 13.95% and 20.48% higher than those at room temperature, respectively, but they decreased obviously compared to those at 43℃. In contrast, the change of the electron acceptor pool of PS II was not obvious compared to those at 43℃, which indicated the electron donor side of PS II was damaged. Moreover, the light harvesting complex (LHCII) in high yield wheat cultivar 01-35 could harvest more sun energy and separated more absorption energy into electron transport than that in Lumai 14, which suggested mat LHCII and the electron transport in high yield wheat cultivar 01-35 could endure a certain higher temperature and maintain higher electron transport capacity than those in Lumai 14.更多还原