【作者】 张晓元； 王松梅； 朱希强； 郭学平； 凌沛学；

【Author】 ZHANG Xiao-yuan, WANG Song-mei, ZHU Xi-qiang,GUO Xue-ping, LING Pei-xue (Institute of Biopharmaceuticals of Shandong Province, Jinan 250108,China)

【机构】 山东省生物药物研究院；

【摘要】 目的研究利用热带假丝酵母发酵木糖生产木糖醇的发酵条件。方法采用摇瓶发酵对发酵生产条件,如培养基中初始木糖浓度、接种量和通气量、氮源、pH等条件,通过测定发酵液中木糖的残留量、木糖醇的转化率来确定较为适合的发酵工艺。结果通过实验得到最佳培养基条件为:初始木糖50 g／L,蛋白胨5 g／L,酵母粉 10 g／L,硫酸镁0.5 g／L、磷酸二氢钾5 g／L、硫酸铵1 g／L;最佳发酵条件为:pH 6．0,摇瓶发酵装液量50 mL／ 250mL,转速200 r／min,发酵温度30℃,发酵时间28 h。结论优化了木糖醇的发酵工艺。更多还原

【Abstract】 Objective This paper researches the fermentation condition of xylitol production utilizing Candida tropicalis. Method An optimal fermentation method for shaken flask culture was achieved by altering culture conditions, such as the initial xylose concentration, inoculum level,aeration, nitrogen source, pH value. The parameters of consequence include content of the residual xylose and the xylitol yield. Results Larger xylitol production was gotten under following medium composition: initial xylose concentration 50g/L,trypton 5g/L, Yeast Extract 10g/L, MgSO40.5 g/L, KH2PO4 5g/L, (NH4)2SO4 1g/L. The optimal culture conditions were: pH 6.0,50ml medium in 250ml shaken flask, rotary rate 200rpm, 30℃ up to 28h. Conclusion Fermentation process of xylitol is optimized.更多还原