【作者】 吕鹏； 庄重； 凌建亚； 张长铠；

【Author】 Peng Lü, Zhong Zhuang, Jianya Ling, Changkai Zhang (State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China)

【机构】 山东大学微生物技术国家重点实验室；

【摘要】 本文对苯酚高效降解菌洋葱伯克霍尔德氏菌L68菌株邻苯二酚2,3-双加氧酶产酶条件进行了正交试验优化。结果表明,最适培养条件是250 ml装液量,2.5%接种量,pH 6.0,2 gl-1的酵母粉和1 gl-1苯酚。通过正交试验,酶产量为0.2154 U/ml,提高了9.25倍。更多还原

【Abstract】 Culture conditions improvement for the production of catechol 2,3-dioxygenase (C23O EC 1.13.11.2 ) by Burkholderia ceoacia L68 was investigated using an orthogonal array design. The experimental results revealed that the volume of medium and the percent of phenol had remarkable effects on the enzyme production, while the percent of inoculation played little role in the production of the enzyme. The improved culture conditions are 250 ml medium consisted of 2 gl-1 yeast extract and 1 gl-1 phenol, pH 6.0, and 2.5 % inoculation. C23O with the improved culture condition production at 48h (about 0.2154 U/ml) was about 9.25-folds greater than that with the control culture conditions.更多还原