【作者】 王正荣； 陈立国； 屈艺； 肖静； 郭慧玲； 万朝敏；

【Author】 LiGuo Chen, Yi Qu, Jing Xiao,HuiLing Guo,ChaoMin Wan, ZhengRong Wang, (1Biomedical Engineering Unit, West china medical center of Sichuan university, 2The second hospital of Sichuan University,Chengdu 610041)

【机构】 四川大学华西医学中心生物医学工程教研室； 四川大学第二医院；

【摘要】 目的本文探讨心房肽(ANP) cDNA转染细胞的微囊化技术, 测定其ANP的表达水平,以及研究ANP分泌的近日节律;通过人工调控,改变ANP分泌的近日节律, 达到有效治疗高血压或心衰的目的。方法将人ANP基因(cDNA)转染入CHO细胞,然后将细胞包被在聚己内酯(PCL)管内形成微囊,并检测转基因CHO细胞长时间存活情况和分泌的ANP。检测微囊化转基因细胞分泌ANP的生物节律,并用褪黑素(melatonin)进行调整。结果在培养期间,长度20mm直径3mm的PCL管内转染CHO细胞在2ml培养基中分泌的ANP水平可达246.1pg/ml/24h;ANP的分泌呈近日节律性变化,日间高,而夜间低;近日节律变化的时相是4:18,用melatonin处理,近日节律的时相移至7:56。结论ANP cDNA转染的CHO细胞包被在PCL管内并能向管外分泌ANP,将来可能适于植入人体。此项研究旨在提供一条心房肽治疗高血压或心衰的新途径。更多还原

【Abstract】 Objective A technique for potential therapeutic approach to hypertension or congestive heart failure (CHF) based on the release of atrial natriuretic peptide (ANP) from encapsulated ANP cDNA transfected Chinese hamster ovary (CHO) cells was investigated. Method ANP cDNA transfected CHO cells were encapsulated in PCL tubes and levels of ANP secreted were detected. Circadian rhythm of ANP secreted by encapsulated transfected cells was also studied by regulation with melatonin. Result During culturing, the encapsulated cells remained viable and the mean level of ANP reached 246.1pg/ml/24h in 2ml medium containing one PCL tube (20mm in length and 3mm in diameter), whereas the secretion by the control was negative. The secretion of ANP showed a circadian variation: higher at night, but lower at day. The acrophase of circadian rhythm was 4:18 and shift to 7:56 after melatonin was given. Conclusion The above results demonstrated a potential use of the encapsulation technique of gene transfected cells implanted into human body for treatment of ANP hypertension or CHF.更多还原