【作者】 张艳珍； 陈细华； 危起伟； 邱实； 刘志刚；

【Author】 ZHANG Yan-zhen~(1,2),CHEN Xi-hua~(2,3),WEI Qi-wei~(2,3),QIU Shi~(1,2),LIU Zhi-gang~(2,3) (1.Huazhong Agricultural University,Wuhan 430070,China;2.Key Laboratory of Freshwater Biodiversity Conservation and Utilization,Ministry of Agriculture of China,Yangtze River Fisheries Research Institute,Chinese Academy of Fisheries Science,Jingzhou 434000,China;3.Freshwater Fisheries Research Center,Chinese Academy of Fishery Science,Wuxi 214081,China)

【机构】 华中农业大学； 农业部淡水生物多样性保护与利用重点开放实验室,中国水产科学研究院长江水产研究所； 中国水产科学研究院淡水渔业研究中心；

【摘要】 中华鲟(Acipenser sinensis)在海水中生长发育,在淡水中产卵繁殖。通过碱不稳定性蛋白结合磷法测定不同年龄中华鲟血清中磷含量,以磷的含量变化反映卵黄蛋白原(VTG)变化情况。实验鱼为人工养殖0.5、1、2、3、4、5、6、8、9、10龄中华鲟和野生成熟中华鲟,共81尾,其中8尾野生雌鱼在人工繁殖前后各取样2～4次,共测97个样本。结果显示低龄中华鲟VTG含量较低,最低值在5龄,含磷量为(4.6±1.6)mg/L。5龄后增加,成熟后达到最大为52.0±4.5 mg/L。在性腺发育不同时期,雌性个体Ⅱ～Ⅴ期VTG增加,Ⅱ～Ⅲ期增加速度较快,随后变缓,V期达到最高水平,Ⅵ期降低;而雄性个体Ⅱ～Ⅳ期增加量很少,最高为(28.7±12.2)mg/L。繁殖雌鱼产前VTG最高,含磷量为(62.5±8.7)mg/mL,产后低于产前。此外,发现产后鲟鱼卵巢液中含量低于其血清,但高于其他年龄组。本实验暗示着淡水养殖的中华鲟有可能达到性成熟。更多还原

【Abstract】 The Chinese sturgeon,Acipenser sinensis Gray,is an anadromous fish.Sexual maturity in males is reached between 8-18 years and in females between 14-26 years.The yolk proteins of Chinese sturgeon(Acipenser sinensis)are chief supplemental nutrition and energy for embryo development, which come from vitellogenin(VTG).The levels of plasma VTG were performed by an indirect method,measuring the alkali-labile phosphorus content of the lipid-free protein fraction.The levels of phosphorus can reflect the VTG levels indirectly.In the experiment,the phosphorus levels in serum of Chinese sturgeon were measured by the chemic response with the aqueous trichloroacetic acid(TCA).Ninety-seven serum samples from 81 fish were measured.The fish included the cultured ones,0.5,1,2,3,4,5,6,8,9 and 10 year-old,and the wild matured ones which were caught below Gezhouba Dam in Yangtze River.The results showed that the VTG was low at the early ages from 1 to 5 years,and then increased.The matured wild sturgeons had the highest levels,(52.0±4.5)mg/L.From the gonadal development stageⅡto stageⅥ,the VTG levels of females were increased quickly especially during stageⅡto stageⅢ,the highest level was at stageⅤ,and then descended at stageⅥ.However,it was slowly in males.The phosphorus levels at stageⅣwere (28.7±12.2)mg/L in males.In the artificial spawning fish,the VTG level was higher in prespawning than in postspawning period.It was(62.5±8.7)mg/mL in prespawning.In addition,the results also showed that it was higher in the overian succus of the spawning stock than in the other ages. It was suggested that the cultured Chinese sturgeon in fresh water were likely to be matured.更多还原