【作者】 王长忠； 梁宏伟； 邹桂伟； 罗相忠； 李忠； 田华； 呼光富；

【Author】 WANG Chang-zhong~(1,2),LIANG Hong-wei~2,ZOU Gui-wei~(1,2),LUO Xiang-zhong~2, LI Zhong~2,TIAN Hua~(1,2),HU Guang-fu~(1,2) (1.Fisheries College of Huazhong Agricultural University,Wuhan 430070,China;2.Key Lab of Freshwater Fish Germplasm Resources and Biotechnology Certificated by Ministry of Agriculture,Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences,Jingzhou 434000,China)

【机构】 华中农业大学水产学院； 农业部淡水鱼类种质资源与生物技术重点开放实验室,中国水产科学研究院长江水产研究所； 湖北荆州434000；

【摘要】 本研究对长江中上游2个鲢群体使用39个微卫星标记进行了遗传多样性分析,计算并统计了平均观测等位基因数、平均有效等位基因数、多态信息含量(PIC)、遗传杂合度、Hardy- Weinberg平衡偏离指数、遗传相似系数、遗传距离等遗传参数。结果表明:万州鲢和监利鲢群体所检测微卫星位点的平均观测等位基因数分别为6.128和4.974;平均有效等位基因数分别为4.107和3.395;多态位点百分率分别为100和94.87;39个微卫星标记共有等位基因259个,173个等位基因为两群体所共有;多态微卫星位点的PIC在0.077～0.865之间变动,平均为0.617;两群体所检测位点平均观测杂合度为0.834和0.775,平均期望杂合度为0.713和0.623;两个群体间的遗传相似系数为0.618,群体间的遗传距离为0.482。结果显示长江中上游两个鲢群体间存在显著遗传分化,应隶属于不同的种群。更多还原

【Abstract】 Thirty nine microsatellite markers were applied to analyze the genetic diversity of two silver carp populations in the middle and upper reaches of the Yangtze River.The following parameters were calculated:average observed number of alleles,average effective number of alleles,genetic heterozygosity, polymorphism in formation content(PIC),genetic similarity coefficient,genetic distance, Hardy-Weinberg balance deflection index.These results indicated that the average observed number of alleles in each microsatellite locus of the Wanzhou population and Jianli populations is 6.128 and 4.974,respectively;the average effective number of alleles is 4.107 and 3.395,respectively; and the number of total alleles of these 39 microsatellite loci is 259.The PIC of polymorphic loci varies between 0.077-0.865,and the average PIC is 0.617.The average observed heterozygosity (H_o)of two populations is 0.834 and 0.775,respectively and the average expective heterzygosity (H_e)is 0.713 and 0.623,respectively.The genetic similarity coefficient of two populations is 0.618 and the genetic distance of these populations is 0.482.These results indicated that the two populations belong to different populations for the obvious genetic differentiation.更多还原