【作者】 葛金文； 朱惠斌； 贺蓉； 王宇红； 贺石林；

【Author】 Ge Jinwen~1 Zhu Huibin~1 He Rong~(2△)Wang Yuhong~1 He Shilin~2 (1Hunan University of Chinese Medicine Changsha 410208;2Central South University School of Medicine Changsha 410008)

【机构】 湖南中医药大学； 中南大学湘雅医学院；

【摘要】 血清的形成与凝血及其相关过程(纤溶与补体系统活化)的蛋白酶级联反应以及血小板和白细胞活化有关,这些过程直接影响血标本的组成,并可能也影响中药成分;但这些过程可用抗凝剂而加以避免。因此,血浆的组成较血清能更好地反映生理、病理状况和吸收进入循环血中药物变化。根据已有报道,药物浓度与反应物质在血浆与血清中可能存在差异,故倡导在中药半体内实验中采用血浆药理学方法。由于不同抗凝剂的作用机制各异,究竟哪一抗凝剂适用于哪类半体内实验,需要进一步开展研究。我们建议在大多数中药半体内实验中,可以采取含药血浆与含药血清平行观察,除非事先对该试验的干扰已有充分了解。更多还原

【Abstract】 The generation of serum is associated with the protease cascades of coagulation, fibrinolysis and complement systems and the activation of platelets and leukocytes.Such processes that directly effect the composition of blood samples and possibly also affect the components of traditional Chinese medicine(TCM)can be avoided by using anticoagulants.Therefore the constituents in plasma are better reflecting the physiological/pathological situation and the changes of drugs absorbed into circulating blood than in serum.Based upon the reports published,the concentrations of drugs and the response substances to them in plasma could be different to those in serum;plasma pharmacological method in ex vivo experiments for TCM studies has been proposed. Because the action mechanisms of anticoagulants are different,the issues that which is indicated in what kind(s)of the ex vivo experiments need to be further research.We suggest that plasma and serum contained the components of TCM are used parallel to each other in most experiments except the interference is known in advance.更多还原