【作者】 姜建兰； 邱一华； 彭聿平；

【Author】 JIANG Jian-lan, QIU Yi-hua, PENG Yu-ping(Department of Physiology, School of Basic Medical Sciences, Nantong University, Nantong 226001, China)

【机构】 南通大学基础医学院生理学教研室；

【摘要】 目的:观察乙酰胆碱(ACh)对自然杀伤(NK)细胞活性的影响,并初步探讨其作用的受体机制。方法:根据不同的实验目的,选择ACh、胆碱能受体激动剂和拮抗剂分别作用于NK细胞,以乳酸脱氢酶(lactatedehydroge-nase,LDH)自然释放法检测不同实验条件下NK细胞杀伤肿瘤靶细胞(Yac)的活性。结果:ACh、M受体激动剂毛果芸香碱和N受体激动剂烟碱在10-10~10-6mol/L浓度范围内都能显著抑制NK细胞杀伤肿瘤细胞的活性。M受体拮抗剂阿托品(10-8和10-7mol/L)能完全阻断同浓度ACh抑制NK细胞活性的作用;但N受体拮抗剂筒箭毒碱(10-8和10-7mol/L)不能阻断同浓度ACh抑制NK细胞活性的作用。结论:ACh可抑制NK细胞对肿瘤细胞的杀伤作用,此作用主要由淋巴细胞上的M受体和N1受体介导。更多还原

【Abstract】 Aim: To investigate the effect of acetylcholine（ACh） on the cytotoxicity of natural killer（NK） cells and to explore the receptor mechanisms involved in the effect. Methods: The effector cells（i.e. NK cells） from the spleens of rats were collected and cultured with the target cells（Yac-1 cells）. The various concentrations of ACh, cholinergic receptor agonists or antagonists were added to the cultures, respectively according to distinct experimental purposes. Lactate dehydrogenase（LDH） release assay was used to evaluate NK cell cytotoxicity. Results: NK-cell-mediated lysis of Yac-1 lymphoma cells was reduced by 10^-10～10^-6 mol/L ACh. The inhibitory effect of ACh on NK cell cytotoxicity was mimicked by pilocarpine, an agonist of muscarinic receptor, and by nicotine, an agonist of nicotinic receptor, at all applied concentrations(10^-10～10^-6 mol/L). Muscarinic receptor antagonist atropine blocked the inhibitory effect of ACh on the cytotoxicity of NK cells. Nevertheless, tubocurarine, an antagonist of nicotinic receptor, had no blocking effect on the suppression of NK cell cytotoxicity by ACh. Conclusion: ACh results in an inhibition of the cytotoxicity of NK cells, and this inhibition is realized mainly through M and N₁ cholinergic receptor.更多还原