【作者】 毛晓波； 曾秋棠； 王祥； 曹林生；

【Author】 MAO Xiao-bo, ZENG Qiu-tang, WANG Xiang, CAO Ling-sheng(Department of Catheter Intervention Cardiology, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China)

【机构】 华中科技大学同济医学院附属协和医院心内科导管室；

【摘要】 目的:通过研究不同时期心肌梗塞区血管生长因子的表达,探讨骨髓基质细胞移植促进心肌梗塞后血管新生的机制。方法:将急性心肌梗塞大鼠随机分为2组。实验组在梗塞后28d,将同种异体骨髓基质细胞注射到心肌梗塞区。对照组仅注射无血清的培养液。在梗塞后的不同时期取标本动态观察梗塞区VEGF、bFGF的表达和血管新生状况。结果:骨髓基质细胞移植入梗塞区后主要分化为成纤维细胞和血管内皮细胞。实验组心肌梗塞区新生毛细血管数目较对照组明显增加(14±4.7/HPFvs6±2.4/HPFP<0.05)。对照组梗塞区VEGF和bFGF的表达在梗塞后7d达高峰,28d开始下降,第42d和56d时表达明显下降。而实验组二者的表达在心肌梗塞后第42d和56d明显高于对照组。结论:骨髓基质细胞通过分化为内皮细胞以及促进梗塞区VEGF和bFGF的持续高表达,对血管新生起积极作用。更多还原

【Abstract】 Aim: To study the mechanism of the therapeutic angiogenesis effect of bone marrow stromal cells(BMSCs) implantation on rat acute myocardial infarction models. Methods: The rat acute myocardial infarction models were made by coronary artery ligation and divided into 2 groups at random. In the experiment group, twice passaged BMSCs were labeled with BrdU and then implanted into the infarction region of the recipients in 4 weeks. The control group was the model rats received only DMEM injection. In control group, the hearts were harvested on the day 3, 7,14,28,42 and 56. The infarction regions were examined to identify the angiogenesis and the expression of the VEGF and bFGF. In experiment group, the hearts were examined on the day 42 and 56 after AMI (the day 14 and 28 after cells implantation). Results: Viable cells labeled with BrdU could be identified in host hearts. Histologic examination found most donor cells within the infarction region expressed fibroblastic and endothelial phenotype. The transplantation regions had a greater capillary density than the control regions did (14±4.7/HPF vs 6±2.4/HPF, P<0.05). In the control group, the expression of VEGF and bFGF within the infarction regions peaked on day 7, and then decreased over time. In the experiment groups, the expression of bFGF and VEGF on the day 42 and 56 had a higher level than the control group did. Conclusion: The expression of VEGF and bFGF is significantly increased after cells therapy during the late phase of AMI. It indicates that BMSCs implantation promoted the angiogenesis is mediated by its differentiation into endothelium and the increased release of VEGF and bFGF.更多还原