【作者】 王慧； 高建刚； 王树迎；

【Author】 WANG Hui1,GAO Jian-gang2 (1.Department of Fisheries,College of Animal Science &Technology,Shandong Agricultural University,Tai’an 271018, China;2.College of Life Science,Shandong University,Ji’nan,Shandong;250100,China)

【机构】 山东农业大学动物科技学院水产系； 山东大学生命科学院；

【摘要】 采用甲基化敏感扩增多态性(Methylation sensitive amplification polymorphism, MSAP)技术,检测了人工养殖的泰山赤鳞鱼全同胞子一代个体的甲基化位点差异。8对引物组合共扩增出127个位点,其中甲基化位点有97个,占总扩增位点的76.37%,表明:泰山赤鳞鱼仔鱼期基因组的甲基化水平较高。其中甲基化多态位点21个,占甲基化位点的21.65%,说明:全同胞个体的甲基化位点有较大差异,这可能与一些性状在后代中的分离和部分基因表达调控的改变有关。更多还原

【Abstract】 Methylation sensitive amplified polymorphism(MSAP) was used to analyze the difference of methylation status between full-sib individuals of Tai’shan Chi-lin fish (Varicorhinus macrolepis).With 8 primer combinations,127 amplified loci were identified from the family collected in 2006,of which 97 loci(about 76.37%) were methylated, suggesting a high methylation level.21.65%of the methylated loci were polymorphic, implying that the expression of some genes might be differentially expressed among these full-sib individuals.The results indicate that different offsprings of the same parents display differential methylation patterns.It also show that phenotypic changes may be related to the differential methylation and ultimately affects on the gene expression.更多还原