【作者】 程洁； 赵柏淞； 王斌； 胡景杰； 包振民；

【Author】 Jie Cheng,Bosong Zhao,Bin Wang,Jingjie Hu,Zhenmin Bao~* Lab of Molecular Genetics and Breeding of mollusk,College of Marine Life Science,Ocean University of China,Qingdao 266003,People’s Republic of China

【机构】 中国海洋大学海洋生命学院贝类分子遗传学与育种技术研究室；

【摘要】 栉孔扇贝(Chlamys farreri)是我国北方最重要的海水经济养殖贝类之一,在海水养殖产业中占有极其重要的地位。随着分子标记辅助育种在遗传改良上的应用,构建栉孔扇贝的遗传连锁图谱和物理图谱以及最终实现遗传图谱与物理图谱的整合,都是栉孔扇贝基因组学研究的重要内容。本实验室2007年成功构建了第一个栉孔扇贝fosmid文库以及微卫星的遗传连锁图谱。本文通过构建文库的三维两步PCR筛选系统,选取实验室已开发的150个微卫星标记对文库进行筛选和分析,结果总共得到含有微卫星标记的fosmid克隆250个,与实验室构建的SSR遗传连锁图谱比对,其中有120个标记分布于微卫星连锁图谱19个连锁群上,共200个阳性克隆与连锁图谱上的微卫星标记关联,实现了fosmid克隆与遗传连锁图的整合。同时,我们构建了一个包含fosmid克隆、与其相关联的微卫星位点、扩增引物与PCR条件等相关信息的数据库。上述研究,为栉孔扇贝细胞遗传图和物理图的构建以及遗传连锁图和物理图的整合奠定基础。更多还原

【Abstract】 To apply genome-based technologies for genetic improvements using marker-assisted selection,genome research involving genetic linkage mapping and physical mapping is required,and integration of genetic and physical maps would significantly enhance the capacities for genome research.In Zhikong scallop(Chlamys farreri),the major aquaculture species in the China,the first large-insert fosmid library and microsatellite linkage map have been constructed in MGB.The first Zhikong scallop fosmid library was used to construct the three-dimensional PCR screening system,and the library was screened with 150 microsatellite markers,of which 120 microsatellites had been mapped to 19 linkage groups of the first microsatellite-based linkage map.As a result,250 clones were isolated with microsatellites and 200 positive clones were anchored on the linkage map. At last we constructed a searchable database for fosmid clones,their linked microsatellites, microsatellite primers,PCR conditions.This will serve as a useful resource for connecting linkage group to its chromosome through hybridizing the chromosome with fosmid clones and for physical mapping,and then the integration of the physical map and genetic map of Zhikong scallop.更多还原