【作者】 杨广； 陈玲； 刘玉兰；

【Author】 Yang Guang Cheng Ling Liu Yulan Department of Fishery Science,Tianjin Agricultural college Key Laboratory of Aquatic Ecology and Aquaculture of Tianjin Tianjin 300384,China

【机构】 天津农学院水产科学系天津市水产生态及养殖重点实验室；

【摘要】 轮虫休眠卵在盐度为25的水体中(25℃)孵化,以不同浓度梯度的嗜水气单胞菌(活菌)和爱德华氏菌(灭活细菌)作为饵料定时定量投喂轮虫幼体。6d后活菌组中轮虫的最大密度70.3个/mL,而灭活细菌组最大密度为23个/mL。依此调整轮虫最佳投喂量范围,获得轮虫最大日平均增殖率(投喂量1.2×1016个/mL)可达0.377。细菌染色后投喂轮虫,显微观察细菌在轮虫体内的消化情况以获得消化时间。不同温度条件下,轮虫对细菌的消化时间与温度呈正相关关系,但温度过高时会延长其消化时间;以细菌在消化道内存留时间的1/3～2/3作为破碎时间,检测轮虫摄食细菌强度,投喂嗜水气单胞菌后8～15 min轮虫体内细菌数为26785.3个,而爱德华氏菌16～32 min的数量为3652.2个。更多还原

【Abstract】 By the way of one-time incubation and cultivation,the dormancy eggs of Brachionus plicatilis(Rotifer) were hatched in the sea water of 25 salinity at 25℃.Aeromonas hydrophila(live bacteria) and Edwardsiella tarda(inactivated bacteria) were fed to Rotifer with different concentration regularly.After 6 days,the maximal density of Rotifer fed on live bacteria reached 70.3 ind./mL,and 23 ind./mL for inactivated bacteria group.When fed on optimum dose of bacteria(1.2×1016 ind./mL),the maximum daily weight gain of Rotifer reached 0.377.The time bacteria were digested by Rotifer was determined under microscopic by feeding stained bacteria.Positive correlation between the time and temperature was obtained at the different temperature.However,too high temperature would delay the being digested of bacteria.After 1/3～2/3 digesting time,Rotifer was broken and the density of bacteria in the intestine was determined.The results showed there were 26785.3 bacteria in one Rotifer feeding on A.hydrophila during 8～15 minutes after feeding and 3652.2 bacteria in one Rotifer feeding on E.tarda during 16～32 minutes after feeding.更多还原