【作者】 周志刚； 刘玉春； 石鹏君； 何夙旭； 姚斌； E.Ringф；

【Author】 ZHOU Zhigang1~*,LIU Yuchun1~*,SHI Pengjun1,HE Suxu1,YAO Bin1~*,Ringф E.2,3 (1.Key Laboratory for Feed Biotechnology of the Ministry of Agriculture,Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China; 2.Department of Marine Biotechnology,Norwegian College of Fishery Science,University of Tromsф,9037 Tromsф,Norway; 3.Institute of Marine Research,5817 Bergen,Norway.)

【机构】 中国农业科学院饲料研究所农业部饲料生物技术重点实验室； Department of Marine Biotechnology,Norwegian College of Fishery Science,University of Tromsφ,9037 Tromsφ,Norway； Institute of Marine Research,5817 Bergen,Norway；

【摘要】 本研究采用结合16S rDNA PCR的梯度凝胶电泳技术(DGGE)对网箱养殖青石斑鱼包括胃、幽门垂、前肠、中肠及后肠的内源性菌群结构进行分析。DGGE图谱上有22条带成功测序,经鉴定后其中10条带代表未培养细菌,消化道优势内源性菌为Proteobacteria,其它属于Firmicutes、Bacteroidetes、Deinococcus-Thermus、Spirochaetes及无法归类的菌。Pantoea sp.及uncultured Proteobacterium在消化道不同部位均鉴定出来,而Empedobacter sp.PH7-1及Acinetobacter sp.N15仅出现在胃中,同时uncultured bacterium clone F6-37、)γ-Proteobacterium及Acinetobacter radioresistens Philippines-11只出现在前肠、中肠及后肠中。除此以外,本研究还鉴定出Lactococcus lactis、Bacteroides及uncultured Streptococcus sp.。更多还原

【Abstract】 The present study used 16S rDNA PCR denaturing gradient gel electrophoresis (PCR-DGGE) technology to investigate the communities of autochthonous microbiota in the gastrointestinal(GI) tract;including stomach(ST),pyloric caeca(PC),proximal intestine(PI), mid intestine(MI) and distal intestine(DI) of the adult yellow grouper(Epinephelus awoara) cultured in cages.Twenty-two DGGE bands were successfully sequenced.However,ten of these bands were classified as unculturable according to the phylogenetic analysis.The dominant autochthonous bacteria in the GI tract belonged to Proteobacteria,but other bacteria identified belonged to Firmicutes,Bacteroidetes,Deinococcus-Thermus,Spirochaetes and unclassified-bacteria.Pantoea sp.and uncultured Proteobacterium were ubiquitous in all five sections of the GI tract.Empedobacter sp.PH7-1 and Acinetobacter sp.N15 were unique for the ST section,while uncultured bacterium clone F6-37 andγ-Proteobacterium and Acinetobacter radioresistens Philippines-11 were only observed in PI,MI and DI sections. Furthermore,Lactococcus lactis,Bacteroides and uncultured Streptococcus sp.were also detected in the present study.更多还原