【作者】 程光平； 吴庆余； 缪晓玲； 黄卫； 汤娇雯；

【Author】 CHENG Guang-ping~1 WU Jing-yu~2 MIU Xiao-ling~2 HUANG Wei~2 TANG Jiao-wen~2 (1 Fisheries Deparrment,Animal Science and Technology College,Guangxi University,Nanning 530005,China;2 Biology Department,Tsinghua University,Beijing 100084,China)

【机构】 广西大学动物科技学院水产系； 清华大学生物系；

【摘要】 在接种螺旋藻并富含NaHCO₃的Zarrouk培养液中恒流输入CaCl₂,观测Ca²⁺与培养液中的HCO ^-结合所产生的CaCO₃对藻类生长及沉积的影响。试验表明,大于0.100 mol/L的CaCl₂浓度明显抑制甚至终止螺旋藻的生长;沉积物随CaCl₂浓度升高而增加,但两者间非正比例关系,沉积量增加的最佳CaCl₂浓度范围为0.001～0.100 mol/L,当CaCl₂浓度大于0.100 mol/L时,沉积量虽有所增加,但增势趋缓;培养体系中悬液藻与沉积藻在CaCl₂浓度0.037 mol/L、钙盐比0.0568时达到生长与沉积平衡,即悬液中和被沉积CaCO₃埋藏的藻细胞生物量（干重）均为521 mg/L（104 mg/L·d）更多还原

【Abstract】 A simulation experiment was conducted by using zurrouk medium contained rich NaHCO₃ as well as flow-adding CaCl₂ with fixed rate to culture Spirulina platensis（SP）and detected the effect of CaCO₃(produced by the reaction of HCO₃^-and Ca²⁺)on SP deposit.The experiment results were as follows.The SP growth was inhibited and even stop when the concentration of CaCl₂ was over 0.100mol/L. The amount of SP deposit increased with the increase of CaCl₂ concentration.The growth of SP deposit increased quickly when the range of optimum CaCl₂ concentration was from 0.001 mol/L to 0.100 mol/L. The amount of SP deposit also increased when the CaCl₂ concentration was over 0.100 mol/L,but the increase was very slow.Suspending SP and deposited SP reach balance and the dry weight of both Suspending SP and deposited SP was 521mg/L when the CaCl₂ concentration and ratio of Ca²⁺to total mineral were 0.037 mol/L and 0.0568,respectively.更多还原