【作者】 徐为中； 薛家宾； 诸玉梅； 陈兴祥； 周永银； 杨龙圣； 尹秀凤；

【Author】 XU Wei-zhong~1 2,XUE Jia-bin~1 ZHU Yi-mei~1 CHEN Xing-xiang~1 ZHOU Yong-yin~1 Yang Long-sheng~2 Yin Xiu-feng~1 (1 Institute of Veterinary Medicine,Jiangsu Academy of Agriculture Science,Nanjing,Jiangsu 210014,China; 2 College of Veterinary Medicine,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China)

【机构】 江苏省农科院兽医研究所； 南京农业大学动物医学院；

【摘要】 采用超纳滤技术浓缩家兔产气荚膜梭菌(A)型疫苗,建立家兔产气荚膜梭菌(A)型疫苗浓缩工艺。配制兔产气荚膜梭菌(A)型超纳滤浓缩疫苗,并对其无菌性、安全性和免疫效力进行了检验。抗原浓缩7倍处理。结果显示,用 NaOH 洗后,超纳滤膜的通量可以恢复；配制的家兔产气荚膜梭菌(A)型超纳滤浓缩疫苗无菌检验合格,安全检验家兔无不良反应,符合动物用生物制品要求；以2mL/只的剂量免疫家兔,家兔产气荚膜梭菌(A)型超纳滤浓缩疫苗的保护率达100%,传统铝胶疫苗对照组的保护率平均为86．7%,表明超纳滤浓缩疫苗免疫效力优于传统铝胶疫苗。确定了家兔产气荚膜梭菌病(A)型疫苗超纳滤膜膜浓缩的工艺。更多还原

【Abstract】 Studies were made on Concentration of inactived vaccine against clostridium perfringens Type A of Rabbits by ultrafiltration technology.Techniques for uhrafiltration membrane Concentration of inactived vaccine against clostridium perfringens type A of rabbits was established.The antigen was condensed 7 times by ultrafiltration membrane.The vaccine which was made by the condensed antigen was measured by asepsis,safty and effectiveness.It is showed that the flux of filter could be resumed after the use of NaOH. The aseptic examination is qualified,The rabbits behaved no bad reaction by Safe examination,as well as it can provided 100% protection when injected with 2ml vaccine,in addition,the vaccine of control group provides 86.7% protection..The mensuration of effectiveness showed that the vaccine produced by ultrafiltration membrane was better than traditional aluminium vaccine.so the technique of ultrafiltration membrane clostridium Perfringens type A is confirmed.更多还原