【作者】 张朝军； 李付广； 刘传亮； 武芝霞； 王玉芬； 李凤莲；

【Author】 ZHANG Chao-jun, LI Fu-guang, LIU Chuan-liang, WU Zhi-xia, WANG Yu-fen, LI Feng-lian (Cotton Research Institute of CAAS, Key Laboratory of Cotton Genetic Improvement, Ministry of Agriculture, Anyang Henan 455004, China)

【机构】 中国农科院棉花研究所农业部棉花遗传改良重点开放实验室；

【摘要】 实验从激素、氮源等方面研究,最终认为氮源配比不合适是主要诱因,与KT、6-BA、IAA等激素关系不明显;经过对MS培养基的改良,一次继代就可以获得绿色胚状体,从而缩短了组织培养周期近2个月。更多还原

【Abstract】 In cotton tissue culture, normal green embryoid obtained difficultly after 3 or 4 times subcultures because the callus usually turned brown in the subculture from EC to embryoid. The brown-turning was mainly result from the improper ratio of nitrogen after our researches on hormones, nitrogen resource, etc. It was also indicated that the content of KT,6-BA and IAA had no significant effect on embryo tissue brown-turning, the normal green embryoid was produced only by once subculture after improvement of MS culture medium, shorten its cycle about two months.更多还原