【作者】 赖家业； 刘凯； 石海明； 白林含； 尹承颖； 韦鹏霄； 岑秀芬； 罗荡平；

【Author】 LAI Jia-ye~(1,2),LIU Kai~1,SHI Hai-ming~1,BAI Lin-han~2,YIN Cheng-ying~3,WEI Peng-xiao~4, CEN Xiufeng~4,LUO Dang-ping~1 (1.Key Laboratory of Fast-Growing Tree Breeding and Cultivation in Central South China of Ministry of National Forestry,Guangxi University,Nanning,530004,China; 2,College of Life Science,Sichuan University,Chengdu,610064,China;3.College of Forestry,Sichuan Agriculture University,Yaan,625014,China;4.College of Agriculture, Guangxi University,Nanning,530004,China)

【机构】 广西大学国家林业局中南速生材繁育重点实验室； 四川大学生命科学学院； 四川农业大学林学院； 广西大学农学院；

【摘要】 采用根癌农杆菌介导法进行nptⅡ基因对广西桉树优良主栽品种尾叶桉无性系3228(Clone 3228 of Eucalyptus urophylla)茎段的遗传转化研究,对转化过程中的一些因素进行了探讨:桉树茎段对卡那霉素的敏感实验,得出最适筛选浓度为75mg/L,头孢噻肟钠的最适抑菌浓度为300mg/L;2-4min是最适合叶片侵染的时间;共培养2d对茎段转化较适宜,得到抗卡那霉素的抗性芽的频率为2.6%。更多还原

【Abstract】 stem of Clone 3228 of Eucalyptus urophylla containing nptⅡwas investigated and Agrobacterium tumifaciens-mediated transformation was used.Factor on Agrobacterium tumifaciens-mediated gene transfer was discussed indetail.The optimal concentration of kanamycin to select was 75mg/L and of cefotaxime sodium to strain fungus was 300mg/L.The pre-cultivation was unnecessary.The ideal time for stem dipping in Agrobacterium was 2-4 min and the optimal co-cultivation was 2 days.The ratio of Kanamycin-resistant shoots was 2.6%.更多还原