【作者】 袁景淇； 任海涛； 王明珠； 任乐民；

【Author】 YUAN Jing-qi, REN Hai-tao, WANG Ming-zhu,REN Le-min ( Department of Automatic Control, Shanghai Jiaotong University, Shanghai 200030; State Key Laboratory of Bioreactor Engineenng, East China University of Science and Technology, Shanghai 200237; New drug research and development center, North China Pharmaceutical Corporation, Shijiazhuang 050015)

【机构】 上海交通大学自动化系； 华北制药集团新药研究开发中心；

【摘要】 本文基于甲醇营养型重组毕氏酵母Pichia pastoris发酵过程的动力学模型,设计并实施了甘油补料期的细胞同步生长控制和甲醇流加期的比生长速率控制。实验结果表明,模型预测控制优于该菌株的标准补料控制策略。更多还原

【Abstract】 A structured kinetic model for the recombinant yeast, Pichia pastoris, expressing recombinant human serum albumin (rHSA) was used to uetermine the feeding strategies of glycerol and methanol. The model-based glycerol feeding profile is aimed at synchronizing the cell population while keeping the biomass concentration at the desired high level. The feeding profile of methanol is to keep constant specific growth rate during production phase. Experimental results indicated, the model based feeding strategies outperform the standard protocols in the sense of maximizing productivity.更多还原